For now just consider them paired-end fastq files to be processed. These fastq files were generated by 2x250 Illumina Miseq amplicon sequencing of the V4 region of the 16S rRNA gene from gut samples collected longitudinally from a mouse post-weaning.
To follow along, download the example data and unzip. The data we will work with are the same as those used in the mothur MiSeq SOP. Older versions of this workflow associated with previous release versions of the dada2 R package are also available: 1.6, 1.8, 1.12. If you don’t already have it, see the dada2 installation instructions.
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